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human embryonic microglial cells  (CLS Cell Lines Service GmbH)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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microglial cells  (ATCC)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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mouse microglial cell line bv2  (ATCC)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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hmc3 human microglial cells  (ATCC)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
Hmc3 Human Microglial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microglial cells  (Procell Inc)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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hmc3 microglial cells  (ATCC)
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Effects of conditioned media (CM) from fecal LPS‐treated <t>HMC3</t> <t>microglial</t> on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.
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Image Search Results


Effects of conditioned media (CM) from fecal LPS‐treated HMC3 microglial on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.

Journal: Chembiochem

Article Title: Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling

doi: 10.1002/cbic.70557

Figure Lengend Snippet: Effects of conditioned media (CM) from fecal LPS‐treated HMC3 microglial on SH‐SY5Y viability and differentiation. (A) Bright‐field image of differentiated SH‐SY5Y cells (40× magnification, scale bar: 20 µm). (B) Cell viability of SH‐SY5Y neurons after 24 h exposure to CM from HMC3 microglia previously stimulated with 0.1, 1, 10, 100 ng/mL of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). (C) Representative integrated immunofluorescence images combining MAP2 (red) and DAPI (blue) channels, showing MAP2 immunoreactivity in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL fecal. The integration of MAP2 and DAPI channels provides both neuronal morphology and nuclear localization within the same composite image. Original magnification: 10× (scale bar, 100 µm); zoomed‐in image: 40× (scale bar, 20 µm). (D) Quantitative MAP2 immunofluorescence analysis performed using ImageJ software (version 1.53t), MAP2 cells integrated density was expressed as % relative to NS. (E) Western blot analysis of MAP2 protein levels in SH‐SY5Y cells exposed to CM from HMC3 cells treated with 100 ng/mL of fecal LPS. Data were presented as mean ± SEM and NS cells served as negative control. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test.

Article Snippet: Human embryonic microglial cells (HMC3, #300102) and human neuroblastoma cells (SH‐SY5Y, #300154) were purchased from Cytion Biosciences (Heidelberg, Germany).

Techniques: MTT Assay, Immunofluorescence, Morphology, Analysis, Software, Western Blot, Negative Control, Comparison